钩藤碱对甲基苯丙胺依赖小鼠位置偏爱和海马谷氨酸受体1表达的影响

李俊葵; 刘伟; 彭菊; 江明金; 刘怡; 郭友立; 莫志贤

doi:10.13422/j.cnki.syfjx.2014180128

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钩藤碱对甲基苯丙胺依赖小鼠位置偏爱和海马谷氨酸受体1表达的影响

药理 | 更新时间：2024-01-04

- 钩藤碱对甲基苯丙胺依赖小鼠位置偏爱和海马谷氨酸受体1表达的影响
- Effect of Rhynchophylline on Expression of Glutamate Receptor 1 in Methamphetamine-dependent Mice on Conditioned Place Preference
- 中国实验方剂学杂志 2014年20卷第18期页码：128-132
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81173581，81229003）
- DOI：10.13422/j.cnki.syfjx.2014180128
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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李俊葵, 刘伟, 彭菊, 等. 钩藤碱对甲基苯丙胺依赖小鼠位置偏爱和海马谷氨酸受体1表达的影响[J]. 中国实验方剂学杂志, 2014,20(18):128-132.

LI Jun-kui, LIU Wei, PENG Ju, et al. Effect of Rhynchophylline on Expression of Glutamate Receptor 1 in Methamphetamine-dependent Mice on Conditioned Place Preference[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 128-132.
李俊葵, 刘伟, 彭菊, 等. 钩藤碱对甲基苯丙胺依赖小鼠位置偏爱和海马谷氨酸受体1表达的影响[J]. 中国实验方剂学杂志, 2014,20(18):128-132. DOI： 10.13422/j.cnki.syfjx.2014180128.

LI Jun-kui, LIU Wei, PENG Ju, et al. Effect of Rhynchophylline on Expression of Glutamate Receptor 1 in Methamphetamine-dependent Mice on Conditioned Place Preference[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 128-132. DOI： 10.13422/j.cnki.syfjx.2014180128.

摘要

目的：观察钩藤碱对甲基苯丙胺依赖小鼠位置偏爱和海马谷氨酸受体1（GluR1）蛋白表达的影响。方法：随机将50只小鼠分为5组：空白对照组，甲基苯丙胺模型组（4 mg·kg-1），氯胺酮（15 mg·kg-1）组，钩藤碱低剂量（40 mg·kg-1）组，钩藤碱高剂量（80 mg·kg-1）组，每组每天给予相应的药物，连续4 d，建立小鼠位置偏爱模型；免疫组化法和Western blot法检测GluR1蛋白的表达。结果：与正常对照组比较，模型组小鼠在伴药箱中逗留的时间明显增多（P<0.01），成功复制甲基苯丙胺依赖的小鼠位置偏爱模型，与模型组比较，氯胺酮组、钩藤碱低、高剂量组均可以明显减少小鼠在白箱逗留的时间（P<0.01）。免疫组化结果显示，甲基苯丙胺模型组与正常对照组相比蛋白GluR1表达明显增强（P<0.01），而与模型组比较，氯胺酮组、钩藤碱低剂量和高剂量组蛋白GluR1表达减少（P<0.01）；Western blot结果显示，与正常对照组比较，模型组GluR1蛋白表达量明显上调（P<0.01），与模型组比较，氯胺酮组、钩藤碱低剂量和高剂量组GluR1蛋白表达下调（P<0.01）。结论： GluR1蛋白在甲基苯丙胺依赖小鼠位置偏爱形成中发挥着重要作用。

Abstract

Objective: To study the effect of rhynchophylline(Rhy) on glutamate receptor 1 (GluR1) subunit in hippocampus of methamphetamine-induced conditioned place preference (CPP)mice. Method: Mice were randomly divided into five groups randomly with 10 mice in each group:normal control group

methamphetamine model group

methamphetamine with Rhy (40 mg·kg-1)

methamphetamine with Rhy (80 mg·kg-1)

methamphetamine with ketamine (15 mg·kg-1). Each respective drug was administered daily for 4 days. Established place preference mice models were induced by methamphetamine

to observe the expression of GluR1 was observed by immunohistochemistry technique and Western blot. Result: Compared with normal control group

the time of mice with methamphetamine model group in non-preferred compartment increased significantly (P<0.01)

indicating that methamphetamine place preference model was successfully established. Compared with methamphetamine model group

ketamine (15 mg·kg-1)

Rhy (40 mg·kg-1)and Rhy (80 mg·kg-1) reduced the time spent on white compartment of CPP mice significantly(P<0.01).Immunohistochemistry showed that the expression of GluR1 in hippocampus was increaseding in the methamphetamine model group(P<0.01)

while in the ketamine group

low and high doseage Rhy were decreasing(P<0.01);Western blot showed that the express of GluR1 protein was significantly increased in the model group(P<0.01)

while in the ketamine group

low and high doseage Rhy group were significantly decreasing(P<0.01). Conclusion: GluR1 protein plays an important role in the formation of methamphetamine-induced place preference in mice.

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