Objective: To prepare Prpnephrium triphyllum-Rheum officinale solid lipid nanoparticles and investigate its morphology. Method: HPLC was adopted to determine contents of emodin and triphyllin A with mobile phase of acetonitrile-0.1% formic acid-water for gradient elution and detection wavelength at 226 nm.Film-ultrasonic wave dispersion technique was used to prepare P. triphyllum-R.officinale solid lipid nanoparticles

on the basis of single factor tests

orthogonal test was employed to optimize preparation technique by taking entrapment efficiencies of emodin and triphyllin A as comprehensive evaluation index. Result: Optimal formulation was as following:extract of P. triphyllum-R.officinale 150 μL

stearic acid-soybean lecithin (1: 1)

tween-80 of 1.0%;mean particle size diameter was (301±2.8) nm

Zeta potential was (-19.94±3.82)mV

entrapment efficiencies of triphyllin A and emodin was 61.8% and 81.2%

respectively. Conclusion: These prepared P. triphyllum-R.officinale solid lipid nanoparticles show spherical

this preparation process is stable and feasible.