Objective: To improve the standard of quality control of Mailuotong Jiaonang. Method: TLC identify Salviae miltiorrhizae radix et rhizoma

Crataegi fructus

Chuanxiong rhizome

Pheretima.The Kromasil 100-5C18(4.6 mm×250 mm

5 μm) was column adopted with the temperature at 35 ℃

mobile phase A was methanol

mobile phase B was 1% glacial acetic acid solution(0-45 min

25%-45% A; 45-55 min

45%-25%A)the flow rate at 1.0 mL·min-1

the detection wavelength was 254 nm. Result: The calibration curve of puerarin was linear in the range of 0.130 6-1.959 6 μg with the correlation of 0.999 9(n=9). The average recovery of puerarin was 97.77%

RSD 0.70%(n=6). The calibration curve of rutin was linear in the range of 1.054 2-15.813 6 μg with the correlation of 0.999 9(n=9). The average recovery of rutin was 98.66%

RSD 0.89%(n=6). The calibration curve of salvia acid B was linear in the range of 0.184 9-2.773 2 μg with the correlation of 0.999 5(n=9). The average recovery of salvia acid B was 99.55%

RSD 1.15%(n=6). Conclusion: The method is specific

reliable and accurate

so it can be used for the quality control of Mailuotong Jiaonang.