橘核中柠檬苦素类物质纯化工艺

罗静; 何中燕; 裴瑾; 康亚兰; 刘维; 陈翠平

doi:10.13422/j.cnki.syfjx.2014200013

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橘核中柠檬苦素类物质纯化工艺

工艺与制剂 | 更新时间：2024-01-04

- 橘核中柠檬苦素类物质纯化工艺
- Purification Technology of Limonoids in Citri Reticulatae Semen
- 中国实验方剂学杂志 2014年20卷第20期页码：13-16
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- DOI：10.13422/j.cnki.syfjx.2014200013
  中图分类号： R284
- 纸质出版日期：2014
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罗静, 何中燕, 裴瑾, 等. 橘核中柠檬苦素类物质纯化工艺[J]. 中国实验方剂学杂志, 2014,20(20):13-16.

LUO Jing, HE Zhong-yan, PEI Jin, et al. Purification Technology of Limonoids in Citri Reticulatae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 13-16.
罗静, 何中燕, 裴瑾, 等. 橘核中柠檬苦素类物质纯化工艺[J]. 中国实验方剂学杂志, 2014,20(20):13-16. DOI： 10.13422/j.cnki.syfjx.2014200013.

LUO Jing, HE Zhong-yan, PEI Jin, et al. Purification Technology of Limonoids in Citri Reticulatae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 13-16. DOI： 10.13422/j.cnki.syfjx.2014200013.

摘要

目的：优选橘核中柠檬苦素类物质的大孔树脂纯化工艺。方法：通过静态吸附-洗脱试验综合考察16种不同厂家和极性大孔树脂对橘核中柠檬苦素类物质的纯化效果，筛选最佳树脂型号。通过单因素试验考察HPD-722型大孔树脂对柠檬苦素类物质的动态吸附-洗脱条件，包括清洗剂用量、上样液质量浓度、洗脱剂浓度及用量等。结果：HPD-722型大孔吸附树脂对柠檬苦素类物质的纯化效果最佳；最佳纯化工艺为上样液质量浓度在3.503 3~5.403 4 g ·L-1，上样流速1~2 BV ·h-1，样品溶液pH 6，水洗用量1 BV，80%乙醇6 BV为洗脱剂，洗脱流速2 BV ·h-1，洗脱液pH 7；洗脱液中柠檬苦素类物质纯度82.82%，其中柠檬苦素22.53%，诺米林45.90%。结论：采用HPD-722型大孔吸附树脂纯化橘核中柠檬苦素类物质的工艺可行性较高，可有效提高柠檬苦素类物质纯度。

Abstract

Objective: To optimize purification process of limonoids in Citri Reticulatae Semen by macroporous resin. Method: Static adsorption and desorption tests were adopted to select optimal type from 16 kinds of macroporous resin with different manufacturers and polarities.Purification process was optimized by single factor tests. Result: HPD-722 macroporous resin was selected

optimal purification process was as following:the concentration of sample solution between 3.503 3-5.403 4 g·L-1

sampling speed of 1-2 BV·h-1

pH of sample solution 6

washed impurity by 1 BV of water and eluted by 6 BV of 80% ethanol with elution rate of 2 BV·h-1

eluent pH of 7;under these conditions

purity of limonoids was 82.82%

including limonin of 22.53% and nomilin of 45.90%. Conclusion: Limonoids can be purified by HPD-722 macroporous resin

this process can effectively improve the purity of limonoids.

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