Objective: To optimize extraction technology of Wenyang Huoxue granules. Method: HPLC was adopted to determine the content of paeoniflorin with mobile phase of acetonitrile-0.1% phosphoric acid solution (14:86) and detection wavelength at 230 nm;HPLC-ELSD was employed to determine the content of ginsenoside Rg1

Re

Rb1with mobile phase of acetonitrile(A)-water(B) for gradient elution (0-35 min

19%A;35-55 min

19%-29%A;55-70 min

29%A;70-100 min

29%-40%A).With yield of dry extract and total contents of paeoniflorin

ginsenoside Rg1

ginsenoside Re

ginsenoside Rb1 as comprehensive evaluation index

orthogonal design was taken for investigating effects of the amount of water

extraction times and time on extraction technology. Result: Optimal extraction conditions were as followings:reflux extracted thrice with 8 times the amount of water for 1.5 hours each time;yield of dry extract was 39.64%

contents of ginsenoside Rg1

Re

Rb1 and paeoniflorin were 3.49

2.79

6.64

218.60 mg. Conclusion: This optimized technology is reasonable

simple and suitable for industrial production of Wenyang Huoxue granules.