REN Xue-min, XING Hui-cun, LI Jin-na, et al. Analysis of Protein and Amino Acid in the Root and Stalk of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 74-76.
REN Xue-min, XING Hui-cun, LI Jin-na, et al. Analysis of Protein and Amino Acid in the Root and Stalk of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 74-76. DOI: 10.13422/j.cnki.syfjx.2014200074.
Objective: The puepose of this study was to determine the protein content in the root and stalk of Stellera chamaejasma
and to analyze the amino acid composition. Method: The protein content was determined by spectrophotometry with colored by coomassie brilliant blue G-250;the amino acid composition were analyzed by automatic amino acid analyzer. Result: The protein content in the root and stalk of S. chamaejasma were 12.26 mg·g-1 and 3.93 mg·g-1
respectively. There were 14 kinds of amino acids both in the root and stalk of S. chamaejasma
with 13 common amino acids. Of the 14 amino acids
the root contained met but in the absence of ser
and the stalk lacked met
while contained ser. The root contained 6 kinds of essential amino acids for human body
while the stalk contained 5.The amino acid content in the root was in a range of 0.116 4-39.561 3 mg·g-1
while that in the stalk was in a range of 0.026 1-0.469 1 mg·g-1. Conclusion: The contents of protein and amino acid in the root were higher than those in the stalk of S. chamaejasma. So the root has higher medical value than stalk in terms of protein and amino acid content.