ZHANG Yue-hao, GUAN Huan-yu, WANG Yong-lin, et al. Non-alkaloid Constituents from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 114-117.
ZHANG Yue-hao, GUAN Huan-yu, WANG Yong-lin, et al. Non-alkaloid Constituents from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 114-117. DOI: 10.13422/j.cnki.syfjx.2014200114.
Objective: To study the non-alkaloid constituents of Buxus sinica. Method: The compounds from ethyl acetate and n-butanol fractionsof B. sinica were isolated byvarious chromatographic techniques including MCI gel
normal phase silica gel
RP-18 Silica geland Sephadex LH-20.The structures were identified by their physical-chemical properties and spectroscopic analysis. Result: Ten compounds were isolated and identified as 4'
5-dihydroxy-3
3'
6
7-tetramethoxyflavone(1)
4'
5-dihydroxy-3
6
7-trimethoxyflavone(2)
3'
4'
5-trihydroxy-3
6
7-timethoxyflavone(3)
pinoresino(4)
3'-O-demethylepipinoresionl(5)
4-hydroxy-3-methoxycinnamic acid methyl ester(6)
methyl-4-hydroxycinnamate(7)
cleomiscosin A(8)
syringin(9) and isolariciresinol-4-O-β-D -glucopyranoside(10). Conclusion: Compounds 1
4-7
and 9-10 were isolated from this plant for the first time.