Objective: The aim of this study was to ascertain the synergistic analgesic interaction and potential mechanism of sodium ferulate (SF) and ginsenoside Rg1 (Rg1). Method: Mice and rats were divided into SF low

medium and high dose groups

Rg1 low

medium and high dose groups

the combination low

medium and high dose groups and the control groups. After 5 days of intragastric administration

the hot plate test

the acetic acid writhing test and the formalin test were used to observe the analgesic effects of SF

Rg1 and the combination. The whole-cell patch-clamp was used to observe the effects of SF

Rg1 and on transient receptor potential vanilloid 1 (TRPV1). Result: Hot plate 55 ℃

0.6% acetic acid solution 0.02 mL·g-1

5% formaldehyde solution 0.1 mL caused obvious pain responses. The medium and high dose groups of SF

the high dose group of Rg1 and the low

medium and high dose groups of the combination showed good analgesic effects on the three models (P<0.05

P<0.01)

and the effects of the combination were better than SF or Rg1 administrated alone (P<0.05

P<0.01).1 μmol·L-1 capsaicin (CAP) activated TRPV1 and induced specific inward current

SF

Rg1 and the combination inhibited the current and the inhibition of the combination was stronger than SF and Rg1 group (P<0.05

P<0.01). The 50% inhibition concentration (IC50) and 95% confidence interval of SF

Rg1 and the combination were 32.4 (27.5-38.7)

82.6 (71.0-98.4)

71.0 (20.2-41.0) mg·L-1respectively. The isobologram showed that the inhibition of SF and Rg1 on TRPV1 had a synergistic effect. Conclusion: The combination of SF and Rg1 had analgesic effect

the synergistic inhibition of may be one of its mechanisms.