Objective: To optimize extraction and purification procedures of Xiyue granules. Method: HPLC-ELSD was employed to determine the content of astragaloside IV with mobile phase of acetonitrile-water(35:65). HPLC was adopted to determine the content of gentiopicroside by taking methanol-water(27:73) as mobile phase

flow rate was 0.8 mL·min-1 and detection wavelength was 271 nm.Taking composite score of contents of astragaloside IV and gentiopicroside as index

effects of the amount of water

extracting time and times on extraction process was optimized by orthogonal design.Single factor tests were adopted to optimize purification procedure with dry extract rate and contents of astragaloside IV and gentiopicroside as indexes. Result: The best extraction process was as follows:extracted 3 times with 10 times the amount of water for 1.0 h each time;contents of astragaloside IV and gentiopicroside were 4.34

43.7 mg

respectively.Optimum purification process was ethanol precipitation which contained 50% alcohol;contents of astragaloside IV and gentiopicroside

dry extract rate were 21.30 mg

216.32 mg and 22.13%. Conclusion: Optimized extraction and purification processes are stable and reliable

they can provide references for industrial production of Xiyue granules.