Objective: To improve extraction efficiency of DNA in Asini Corii Colla and provide a reference for extracting DNA in other products containing high protein content. Method: DNA in Asini Corii Colla was extracted by SDS method and CTAB method

according to their own characteristics of Asini Corii Colla products

reaction time of proteinase K and protein elution step in SDS method were optimized. Result: Under the same conditions

average extraction capacity of DNA in Asini Corii Colla by SDS was higher than CTAB

whose numerical difference was 0.022 g·L-1.Optimized SDS method was as following:reaction time of proteinase K 1.5 h

eluted protein in Asini Corii Colla by phenol-chloroform-isoamyl alcohol for three times

purity of DNA in Asini Corii Colla increased 48.3% by comparing with the original process. Conclusion: Scavenging effects on protein in Asini Corii Colla by SDS method is higher than CTAB

this optimized SDS method is suitable for extraction of DNA in Asini Corii Colla

it provides conditions for biological detection of animal derived molecules from Asini Corii Colla.