Box-Behnken设计优化石斛清咽含片提取工艺

刘怀伟; 邹俊波; 张廷模; 邹文俊

doi:10.13422/j.cnki.syfjx.2014210017

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Box-Behnken设计优化石斛清咽含片提取工艺

工艺与制剂 | 更新时间：2024-01-04

- Box-Behnken设计优化石斛清咽含片提取工艺
- Optimization of Extraction Technology of Shihu Qingyan Lozenges by Box-Behnken Design-response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第21期页码：17-20
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项(2011BAI13B02-8)
- DOI：10.13422/j.cnki.syfjx.2014210017
  中图分类号： R287;R283.6;R284.2
- 纸质出版日期：2014
- 稿件说明：
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刘怀伟, 邹俊波, 张廷模, 等. Box-Behnken设计优化石斛清咽含片提取工艺[J]. 中国实验方剂学杂志, 2014,20(21):17-20.

LIU Huai-wei, ZOU Jun-bo, ZHANG Ting-mo, et al. Optimization of Extraction Technology of Shihu Qingyan Lozenges by Box-Behnken Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 17-20.
刘怀伟, 邹俊波, 张廷模, 等. Box-Behnken设计优化石斛清咽含片提取工艺[J]. 中国实验方剂学杂志, 2014,20(21):17-20. DOI： 10.13422/j.cnki.syfjx.2014210017.

LIU Huai-wei, ZOU Jun-bo, ZHANG Ting-mo, et al. Optimization of Extraction Technology of Shihu Qingyan Lozenges by Box-Behnken Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 17-20. DOI： 10.13422/j.cnki.syfjx.2014210017.

摘要

目的: 优选石斛清咽含片的提取工艺。方法: 采用HPLC测定石斛酚含量

检测波长278 nm

流动相乙腈(A)-0.5% 甲酸(B)梯度洗脱(0~20 min

27% A;20~30 min

27% ~40% A;30~45 min

40% ~65% A;45~50 min

65% ~27% A)。以石斛酚提取量和干膏率的综合评分为指标

在单因素试验基础上

通过Box-Behnken试验考察提取时间、料液比、浸泡时间对提取工艺的影响。结果: 最佳提取工艺为加11倍量水浸泡35 min

提取3次

每次1 h;石斛酚提取量0.189 6 mg·g-1

干膏得率41.27%。结论: 通过Box-Behnken效应面法及总评“归一值”为评价系统优选的提取工艺合理可行

为石斛清咽含片的应用提供参考。

Abstract

Objective: To optimize extraction process of Shihu Qingyan lozenges by Box-Behnken design-response surface methodology. Method: HPLC was employed to determine the content of dendrophnol with detection wavelength at 278 nm and mobile phase of acetonitrile(A)-0.5% formic acid(B) for gradient elution(0-20 min

27% A;20-30 min

27% -40% A;30-45 min

40% -65% A;45-50 min

65% -27% A).On the basis of single factor tests

a three-factor and three-level Box-Behnken experimental design was employed to investigate effects of extraction time

solid-liquid ratio and soaking time on composite score of extracting amount of dendrophnol and dry extract rate. Result: Optimum process conditions were as follows:soaked 35 min with 11 times the amount of water

extracted thrice for 1 h each time;extracting amount of dendrophnol was 0.189 6 mg·g-1 and dry extract rate was 41.27%. Conclusion: This optimized extraction technology is practical and rational by Box-Behnken design-response surface methodology and overall desirability

it provides a reference for application of Shihu Qingyan lozenges.

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