Objective: To optimize extraction technology of total polyphenols from seeds of Cleome gynandra and evaluate its antioxidant activities. Method: UV was adopted to determine the content of total polyphenols with detection wavelength at 760 nm.Taking the content of total polyphenols as index

selecting solid-liquid ratio

extracting temperature

solvent concentration

extracting time as factors

extraction conditions of total polyphenols were optimized by single factor tests and orthogonal test

its scavenge activity on DPPH·free radical was discussed. Result: The best extraction technology was as follows:extracted 40 min with 20 times the amount of 70% ethanol;the content of total polyphenols was 1.93 mg·g-1.Antioxidative activity test showed that DPPH·scavenging abilities of total polyphenols extract from seeds of C. gynandra and vitamin C had no difference when its concentration of 1.28 g·L-1

they were more than 96%. Conclusion: This optimized ultrasonic extraction technology is stable and feasible

total polyphenols have strong antioxidant activity

this article can provide experimental basis for development and utilization of seeds of C. gynandra.