Objective: To set up a HPLC method for the determination of loganin in Zuogui Wan

Yougui Wan and their disassembled prescriptions. It can provide a quality control standard for Zuogui Wan

Yougui Wan and their disassembled prescriptions. Method: HPLC

a ZOREAX SB-C18 column served as the solid phase

the mobile phase was acetonitrile-water in gradient elution mode. The flow rate was 1.0 mL·min-1 and the sample volume was 20 μL each time

the detection wave length was 240 nm and the column temperature was 30 ℃. Result: The topic establishing method can better determine the content variation of loganin.Both before and after the peak position without impurity peak interference

and the peak was intact.The linear range of loganin was 0.289 0-7.225 0 μg(r=0.999 6).The average recoveries were 99.70%

with RSD of 1.5%.Stability

precision and the rate of sample recovery were all well.According to the results

the content of loganin in common prescription was the highest

while it was low in Zuogui Wan. Yougui Wan and kidney-Yin-nourishing drug has a similar level. Conclusion: The method is simple

accurate and reliable with good reproducibility.It can be used to provide the reference value and guiding significance for Zuogui Wan

Yougui Wan and their disassembled prescriptions' quality control.