Objective: To compare monoester-alkaloids content of different batches of Xiaojin Wan from different manufacturers and provide a reference for clinical drug safety of this preparation. Method: Determination of monoester-alkaloids from Aconiti Kusnezoffii Radix Cocta in Xiaojin Wan was established

including benzoylmesaconine

benzoylaconine and benzoylhypacoitine;mobile phase was consisted of acetonitrile-20 mmol·L-1 sodium dihydrogen phosphate(adjusted pH to 6.5 with triethylamine)(47:53)

detection wavelength was set at 235 nm.The content of these three components in 8 batches of Xiaojin Wan from four manufacturers was compared. Result: Linear ranges of these three components were 4-60 mg·L-1

recoveries were 98.42%

101.79% and 102.13%

respectively.Total content of these three components was up to 214.718 μg·g-1with the lowest value of 18.452 μg·g-1. Conclusion: There is a big difference among 3 kinds of monoester-alkaloids from different manufacturers and different batches of the same manufacturer

which may be related to quality of raw materials

preparation process

production equipment and other factors.