基于UPLC/Q-TOF-MS的“小儿腹泻外敷散”敷脐治疗腹泻大鼠的尿液代谢组学

陈思伟; 陈云明; 刘莉; 刘强; 华何与; 张明慧; 张璐

doi:10.13422/j.cnki.syfjx.2014210127

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基于UPLC/Q-TOF-MS的“小儿腹泻外敷散”敷脐治疗腹泻大鼠的尿液代谢组学

药物代谢 | 更新时间：2024-01-04

- 基于UPLC/Q-TOF-MS的“小儿腹泻外敷散”敷脐治疗腹泻大鼠的尿液代谢组学
- Metabonomic Study on Urine of Diarrheal Rats Treated with Xiaoer Fuxie Waifu Powder Based on UPLC/Q-TOF-MS
- 中国实验方剂学杂志 2014年20卷第21期页码：127-131
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81102823);国家级大学生创新创业训练计划(201312121036)
- DOI：10.13422/j.cnki.syfjx.2014210127
  中图分类号： R284.1;R285.5
- 纸质出版日期：2014
- 稿件说明：
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陈思伟, 陈云明, 刘莉, 等. 基于UPLC/Q-TOF-MS的“小儿腹泻外敷散”敷脐治疗腹泻大鼠的尿液代谢组学[J]. 中国实验方剂学杂志, 2014,20(21):127-131.

CHEN Si-wei, CHEN Yun-ming, LIU Li, et al. Metabonomic Study on Urine of Diarrheal Rats Treated with Xiaoer Fuxie Waifu Powder Based on UPLC/Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 127-131.
陈思伟, 陈云明, 刘莉, 等. 基于UPLC/Q-TOF-MS的“小儿腹泻外敷散”敷脐治疗腹泻大鼠的尿液代谢组学[J]. 中国实验方剂学杂志, 2014,20(21):127-131. DOI： 10.13422/j.cnki.syfjx.2014210127.

CHEN Si-wei, CHEN Yun-ming, LIU Li, et al. Metabonomic Study on Urine of Diarrheal Rats Treated with Xiaoer Fuxie Waifu Powder Based on UPLC/Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 127-131. DOI： 10.13422/j.cnki.syfjx.2014210127.

摘要

目的: 研究小儿腹泻外敷散对番泻叶所致腹泻大鼠尿液代谢组学的影响

确定标志物

阐明治疗机制。方法: 以超高压液相色谱-四级杆飞行时间串联质谱 (UPLC/Q-TOF-MS) 为核心技术

以主成分分析法(PCA)和偏最小二乘判别分析(PLS-DA)为数据解析手段

研究小儿腹泻外敷散对番泻叶所致腹泻大鼠的尿液中内源性代谢产物标志物的浓度影响。结果: 各组之间样本点完全分离

各组内的样本有聚类作用

初步确定了6个生物标记物:马尿酸、柠檬酸、α-酮戊二酸、2-氨基苯甲酸、L-瓜氨酸、N-乙酰-5-羟色胺。小儿腹泻外敷散对6个生物标记物有明显的影响。结论: 从生物体内代谢组学层面确定了小儿腹泻外敷散对番泻叶所致腹泻大鼠具有治疗作用

初步阐明了其治疗机制。

Abstract

Objective: To investigate the effect of Xiaoer Fuxie Waifu powder on the urine of senna-induced diarrheal rats

identify the biomarkers

and explore its therapeutic mechanism. Method: Rat urine was collected and analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). The acquired data were analyzed using principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The concentration changes of endogenous metabolic biomarkers in the urine were studied. Result: Metabolite profiles of the urine samples among groups had been obviously classified. And samples showed clustering character in each group. Six metabolic biomarkers were tentatively identified as urobenzoic acid

citric acid

α-oxoglutarate

2-aminobenzoicacid

L-citrulline and N-acetyl-5-HTA

which could be obviously affected by Xiaoer Fuxie Waifu powder. Conclusion: The administration of Xiaoer Fuxie Waifu powder could affect endogenous metabolites of urine

which may be its therapeutic mechanism.

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