Objective: To study the effects of ethanol componentons from Aconitum vilmorinianum(AVEC) on inflammatory cytokines in lipopolysaccharide(LPS)-stimulated macrophage model. Method: The in vitro model of inflammation model based on the growth of well RAW 264.7 cells was established by treating with LPS (10 μg·L-1) for 24 h. The activities of macrophages were detected by MTT assay. The production of nitric oxide (NO) was assayed by Griess reagent. ELISA were used to assay the production of inflammatory mediators

such as tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in cell supernatant. Result: The cell viability was not significantly affected by up to 400 mg·L-1 of AVEC. Compared with the control group

LPS could induce RAW 264.7 cells to secrete inflammatory mediators like TNF-α

IL-6 and NO (P<0.01). Compared with the model group

100-400 mg·L-1 of AVEC in LPS-stimulated RAW 264.7 cells greatly inhibited the production of inflammatory mediators

such as TNF-α and IL-6 in a good dose dependent manner (P<0.05

P<0.01). Conclusion: AVCE can inhibit LPS-induced inflammatory response in RAW 264.7 cells

and its anti-inflammatory effect may be related to reducing the inflammatory cytokines like TNF-α

IL-6 and NO.