丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化病理、突触素及神经生长相关蛋白的影响

马丙祥; 赵丽娜; 张建奎; 郑宏; 李华伟; 党伟利

doi:10.13422/j.cnki.syfjx.2014210153

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丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化病理、突触素及神经生长相关蛋白的影响

药理 | 更新时间：2024-01-04

- 丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化病理、突触素及神经生长相关蛋白的影响
- Effects of Danshen Injection on Expression of SYP and GAP-43 in Preterm Rats' Periventricular Leukomalacia of Lipopolysaccharide-induced Intrauterine Infection Inflammation
- 中国实验方剂学杂志 2014年20卷第21期页码：153-158
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省重点中医学科(专科)学术带头人培养项目专项课题(2013ZY03010);教育部国家级重点学科、河南中医学院科技创新团队(2010XCXTD08中医药治疗儿科疾病)
- DOI：10.13422/j.cnki.syfjx.2014210153
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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马丙祥, 赵丽娜, 张建奎, 等. 丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化病理、突触素及神经生长相关蛋白的影响[J]. 中国实验方剂学杂志, 2014,20(21):153-158.

MA Bing-xiang, ZHAO Li-na, ZHANG Jian-kui, et al. Effects of Danshen Injection on Expression of SYP and GAP-43 in Preterm Rats' Periventricular Leukomalacia of Lipopolysaccharide-induced Intrauterine Infection Inflammation[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 153-158.
马丙祥, 赵丽娜, 张建奎, 等. 丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化病理、突触素及神经生长相关蛋白的影响[J]. 中国实验方剂学杂志, 2014,20(21):153-158. DOI： 10.13422/j.cnki.syfjx.2014210153.

MA Bing-xiang, ZHAO Li-na, ZHANG Jian-kui, et al. Effects of Danshen Injection on Expression of SYP and GAP-43 in Preterm Rats' Periventricular Leukomalacia of Lipopolysaccharide-induced Intrauterine Infection Inflammation[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 153-158. DOI： 10.13422/j.cnki.syfjx.2014210153.

摘要

目的: 探讨丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化脑组织病理及突触素(synapsin

SYP)、神经生长相关蛋白(growth-protein-43

GAP-43)的调节作用。方法: 采用孕鼠ip脂多糖(lipopolysaccharide

LPS)制备宫内感染/炎症致早产仔鼠脑室周围白质软化动物模型

用生理盐水ip作为正常对照组

生后分别选取模型组与对照组孕鼠子宫、胎盘及仔鼠脑组织进行HE染色

模型成功后

模型组早产仔鼠40只分为丹参注射液高剂量组(A

13.5 g·kg-1)

丹参注射液低剂量组(B

6.75 g·kg-1)

神经节苷脂组(C

6 mg·kg-1)

模型对照组(D

生理盐水9 mL·kg-1)各10只

正常对照组(E

生理盐水9 mL·kg-1)

足月仔鼠10只。各组于生后第8天ip干预至14 d

后同等条件下喂养至21 d

进行神经行为学检测

断头取脑、HE染色、免疫组化法检测SYP

GAP-43的表达。结果: 药物干预组SYP

GAP-43的表达较模型组对照组表达明显升高(P<0.01)

与正常对照组表达明显降低(P<0.01)

模型组的表达较正常对照组表达明显降低(P<0.01)。药物干预组中丹参注射液高、低剂量组、神经节苷脂组SYP

GAP-43表达

三者之间差异无统计学意义。结论: 丹参注射液对宫内感染/炎症致早产仔鼠脑室周围白质软化的作用机制

可能与上调SYP

GAP-43的表达有关。

Abstract

Objective: Investigate the regulation of Danshen injection on intrauterine infection/inflammation in premature rats is periventricular leukomalacia brain pathology and synaptophysin (synapsin

SYP)

nerve growth associated protein (growth-protein-43

GAP-43). Method: Using pregnant rat by intraperitoneal injection of LPS preparation intrauterine infection/inflammation in premature rats periventricular leukomalacia animal models

intraperitoneal injection with saline injection as normal control group

select the model group and the control respectively after birth group of pregnant rats uterus

placenta and brain tissue rats HE staining

after the success of the model

the model 40 preterm rats divided into Danshen high dose group A (13.5 g·kg-1)

Danshen low dose group B (6.75 g·kg-1)

ganglion glucoside grease sodium group C(6 mg·kg-1)

model control group D (9 mL·kg-1) for each 10

normol control group E (normal saline 9 mL·kg-1) full term rats 10

each group in the eighth days after birth to intraperitoneal injection of intervention under 14 days

after 21 days of feeding the same conditions

neurological behavior detection

decapitated

SYP GAP-43 expression

detection HE staining

immunohistochemistry. days

after 21 days of feeding the same conditions

neurological behavior detection

decapitated

SYP GAP-43 expression

detection HE staining

immunohistochemistry. Result: Drug intervention group SYP

GAP-43 expression in the control group compared with the model group was significantly increased (P<0.01)

with the control group was significantly lower (P<0.01). The model group that compared with normal control group expression reduced significantly (P<0.01).Drug intervention group Danshen injection high and low dose group

ganglion glucoside grease sodium group SYP

GAP-43 expression

no statistically significant difference in the three group. Conclusion: Danshen injection of intrauterine infection/inflammation-induced mechanism of preterm rats periventricular leukomalacia

and may raise SYP GAP-43 expression related.

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