补肾活血方对帕金森病大鼠多巴胺能神经元的保护作用及机制

陈松盛; 李琛; 杨颖; 胡春婷; 何娅

doi:10.13422/j.cnki.syfjx.2014210175

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补肾活血方对帕金森病大鼠多巴胺能神经元的保护作用及机制

药理 | 更新时间：2024-01-04

- 补肾活血方对帕金森病大鼠多巴胺能神经元的保护作用及机制
- Protective Effects and Mechanism of Bushen Houxue Fang on Parkinson's Disease Rat Dopaminergic Neurons
- 中国实验方剂学杂志 2014年20卷第21期页码：175-179
- 作者机构：
- 作者简介：
- 基金信息：
  
  2011年陕西省中医管理局项目(2011JC-36)
- DOI：10.13422/j.cnki.syfjx.2014210175
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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陈松盛, 李琛, 杨颖, 等. 补肾活血方对帕金森病大鼠多巴胺能神经元的保护作用及机制[J]. 中国实验方剂学杂志, 2014,20(21):175-179.

CHEN Song-sheng, LI Chen, YANG Ying, et al. Protective Effects and Mechanism of Bushen Houxue Fang on Parkinson's Disease Rat Dopaminergic Neurons[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 175-179.
陈松盛, 李琛, 杨颖, 等. 补肾活血方对帕金森病大鼠多巴胺能神经元的保护作用及机制[J]. 中国实验方剂学杂志, 2014,20(21):175-179. DOI： 10.13422/j.cnki.syfjx.2014210175.

CHEN Song-sheng, LI Chen, YANG Ying, et al. Protective Effects and Mechanism of Bushen Houxue Fang on Parkinson's Disease Rat Dopaminergic Neurons[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 175-179. DOI： 10.13422/j.cnki.syfjx.2014210175.

摘要

目的: 观察补肾活血方对6-羟基多巴胺(6-OHDA)所致帕金森病大鼠黑质多巴胺(DA)能神经元的保护作用。方法: 每只雄性SD大鼠黑质内1次性注射6-OHDA 4 μL(含9.0 μg 6-OHDA和8.8 μg抗坏血酸)制作帕金森病(PD)大鼠模型。分别以不同药物灌胃15 d的方式进行治疗。分为正常对照组(生理盐水 90 mg·kg-1 )

模型组(生理盐水 90 mg·kg-1)、西药美多巴治疗组(美多巴 20 mg·kg-1)、补肾活血方高、中、低(15

5 g·kg-1)6组

通过行为学检测、免疫组化法测定黑质酪氨酸羟化酶(TH)、黑质中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)

观察补肾活血方对DA能神经元的保护作用。结果: ①模型组引起典型的右侧旋转

补肾活血方高剂量组和美多巴组与模型组相比旋转圈数减少有显著性差异(P<0.05)。②美多巴组及补肾活血方高剂量组与模型组比较

黑质SOD活性增高(P<0.05)

GSH-Px活性也增高(P<0.01)

而MDA含量降低(P<0.01)。③TH 免疫组化结果表明

与正常组比较PD模型组神经元数量明显减少(P<0.01)

甚至消失

神经元胞体萎缩

突起不清晰。补肾活血方高剂量组及美多巴组大鼠黑质TH阳性神经元数量较多

胞体较大

突起明显。结论: 补肾活血方对6-OHDA所致帕金森病大鼠黑质DA 能神经元损伤具有保护作用

其作用机制可能是通过抗氧化应激及提高黑质酪氨酸羟化酶水平实现的。

Abstract

Objective: Observe the protective effects of Bushen Houxue fang(BSHXF) on 6-hydroxydopamine (6-OHDA) type of Parkinson's disease in substantia nigra of injection of 6-OHD rat dopaminergic neurons. Method: Substantia nigra once injection of 4 μL 6-OHDA to model the Parkinson's disease rat

and divide those models into 6 different groups: normal control (0.9% NaCl)model (0.9% NaCl)

madopar comparison (20 mg·kg-1 madopar)

high (15 g·kg-1 BSHXF)

mid (10 g·kg-1) and low (5 g·kg-1) dosage of BSHXF. Each group intragastric administration one time every day for 15 days. Using ethology detection

immunohistochemistry to determine substantia nigra tyrosine hydroxylase

SOD

MDA and GSH-Px

then observe the protective effects of Bushen Houxue fang on DA neurons. Result: ①Group model rotates clockwise (11.27±1.68) lap/min

there is significant difference (P<0.05) among High dosage of BSHXF group (6.35± 1.49 lap/min

madobar group (5.76±1.23)lap/min and model group. ②Madobar and high dosage group compare to model group have increasing activity of substantia nigra (P<0.05)

as well as GSH-Px (P<0.01). Meanwhile

high dosage of BSHXF and madobar have similar increase in SOD

GSH-Px and decrease in MDA. ③TH immunohistochemistry result shows BSHXF high dosage and madobar group rat substantia nigra TH have large number of positive neuron

large soma size

significant outgrowth

the comparison of the two groups shows no statistical difference. PD model group has significant decrease in the number of neuron (P<0.01)

even disappearing

neuron soma atrophy

little outgrowth. Conclusion: There are protective effects of BSHXF on 6-OHDA type of Parkinson's disease in substantia nigra of rat dopaminergic neurons

its effect mechanism could achieved through anti-oxidative stress and increase level of tyrosine hydroxylase in substantia nigra.

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