Objective:To optimize matrix formulation of Paridis Rhizoma total saponins cataplasms and establish its determination. Method: With ductility

fabric penetration

uniformity

shaped type

skin following nature

adhesion and repeated exposing paste as comprehensive evaluation indicators

on the basis of preliminary experiments

taking dosages of gelatin

sodium polyacrylate

glycerine and polyvinylpyrrolidone-K30(PVP-K30) as factors

matrix formulation of this preparation was optimized by uniform design.HPLC was adopted to determine contents of polyphyllin Ⅱ and polyphyllin Ⅶ with mobile phase of acetonitrile(A)-water(B) for gradient elution(0-40 min

30%-60%A;40-50 min

60%-30%A;50-55 min

30%A)

flow rate of 1.0 mL · min-1 and detection wavelength at 203 nm. Result: Optimum matrix formulation was gelatin-sodium polyacrylate-PVP-K30-glycerine-glycinate aluminum-azone-total saponins from Paridis Rhizoma(2.0: 4.0: 1.25: 15: 0.16: 1.2: 4);polyphyllin Ⅱ and polyphyllin Ⅶ had good liner relationship in the ranges of 0.055-5.5 μg and 0.086 8-8.68 μg

respectively;contents of them in this cataplasms were 1.06

3.79 mg · g-1

respectively. Conclusion: Prepared cataplasms have great drug loading capacity

good adhesion and appearance.Established HPLC method is convenient

accurate and reliable with good reproducibility

which can be suitable for quality evaluation of this preparation.