Objective:To investigate desalination purification effect of Eupolyphaga enzymatic hydrolysate by DA-201C macroporous resin. Method: Desalination test of Eupolyphaga enzymatic hydrolysate was adopted by DA-201C macroporous resin

effects of pH and concentration of sample solution

type and amount of eluent on Eupolyphaga small peptides were investigated by single factor tests.Folin method was employed to determine the content of small peptides with detection wavelength at 650 nm. Result: Optimum desalination process was as follows:resin-sample solution of 1: 1

the concentration of sample solution for 10 mg · L-1

pH of 7.0

adsorption rate of 1.5 BV · h-1

washed impurity with 3 BV of water and eluted by 3 BV of 75% ethanol;desalination rate was 99.1% with small peptides recovery of 86.05%. Conclusion: This optimized desalination process is simple and feasible

it can achieve desired purpose of Eupolyphaga enzymatic hydrolysate.