Objective:To optimize preparation technology of ligustrazine hydrochloride liposomes which provides a reference for clinical application of ligustrazine. Method: Ligustrazine hydrochloride was quantified by HPLC method

mobile phase consisted of methanol-0.2% triethylamine solution(glacial acetic acid adjusted pH to 5.0)(60: 40) with detection wavelength of 295 nm.Ligustrazine hydrochloride liposomes were prepared by film dispersion method with phospholipid and cholesterol as raw materials

taking encapsulation efficiency as index

orthogonal design was adopted to optimize preparation process with hydration time

weight ratio of cholesterol to phospholipid

weight ratio of ligustrazine hydrochloride to phospholipid and the concentration of phospholipid as factors.encapsulation efficiency

morphology

particle size

Zeta potential and stability of ligustrazine hydrochloride liposomes were investigated. Result: Optimum preparation technology was as follows:hydration time of 30 min

ratio of cholesterol to phospholipid 1: 2

ratio of ligustrazine hydrochloride to phospholipid 1: 15

the concentration of phospholipid 3%;under these conditions

liposomes were nearly spherical with average encapsulation efficiency of (70.27±0.58)%

particle size of (22.9±6.8)nm and Zeta potential of (-29.79±1.24)mV

they were stable under 4 ℃ for 2 weeks. Conclusion: This optimized preparation technology is rational and stable

which can help to improve absorption and prolong effective time.