正交试验优选九龙藤总黄酮纯化工艺

孙瑶; 李春凤; 林炜鑫; 简洁

doi:10.13422/j.cnki.syfjx.2014220029

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正交试验优选九龙藤总黄酮纯化工艺

工艺与制剂 | 更新时间：2024-01-04

- 正交试验优选九龙藤总黄酮纯化工艺
- Orthogonal Design for Optimizing Purification Process of Total Flavonoids from
- 中国实验方剂学杂志 2014年20卷第22期页码：29-32
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西自然科学基金项目(2012GXNSFAA053148)
- DOI：10.13422/j.cnki.syfjx.2014220029
  中图分类号： R283.6;R284.1;R284.2;R282.71
- 纸质出版日期：2014
- 稿件说明：
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孙瑶, 李春凤, 林炜鑫, 等. 正交试验优选九龙藤总黄酮纯化工艺[J]. 中国实验方剂学杂志, 2014,20(22):29-32.

SUN Yao, LI Chun-feng, LIN Wei-xin, et al. Orthogonal Design for Optimizing Purification Process of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 29-32.
孙瑶, 李春凤, 林炜鑫, 等. 正交试验优选九龙藤总黄酮纯化工艺[J]. 中国实验方剂学杂志, 2014,20(22):29-32. DOI： 10.13422/j.cnki.syfjx.2014220029.

SUN Yao, LI Chun-feng, LIN Wei-xin, et al. Orthogonal Design for Optimizing Purification Process of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 29-32. DOI： 10.13422/j.cnki.syfjx.2014220029.

摘要

目的: 优选D101型大孔树脂纯化九龙藤总黄酮的工艺条件

为该成分的工业制剂开发提供参考.方法: 以总黄酮纯度及回收率为评价指标

在单因素试验基础上

采用正交试验考察上样液质量浓度、洗脱剂浓度、洗脱速度及上样液pH对九龙藤总黄酮大孔树脂纯化工艺的影响.采用UV测定黄芩苷含量

检测波长278.5 nm.结果: 最佳纯化工艺为上样液质量浓度15.5 g ·L-1

上样液pH 2~3

最大上样量16.85 mg ·g-1

上样速度0.125 BV ·h-1

径高比1:6

加60%乙醇4 BV以1 BV ·h-1洗脱.总黄酮纯度48.95%

回收率96.08%.结论: 优选的纯化工艺稳定可行

有效提高了九龙藤总黄酮纯度及回收率

适用于九龙藤总黄酮的纯化.

Abstract

Objective:To optimize purification process of total flavonoids from Bauhinia championii by D101 macroporous resin and provide a reference for preparations development of these components. Method: With purity and recovery of total flavonoids as indexes

based on single factor tests

orthogonal design was used to optimize purification process by taking eluent concentration

elution rate

pH and concentration of sample solution as factors.UV was employed to determine the content of baicalin with detection wavelength at 278.5 nm. Result: Optimal purification process was as following:the concentration of sample solution 15.5 g · L-1

pH of sample solution 2-3

the maximum sample volume of 16.85 mg · g-1

sampling rate of 0.125 BV · h-1

diameter and height ratio of 1: 6

eluted by 4 BV of 60% ethanol with elution speed of 1 BV · h-1.Under this process

purity and recovery were 48.95% and 96.08%

respectively. Conclusion: This optimized purification process is stable

feasible and suitable for purifying total flavonoids from B. championii

which can effectively improve its purity and recovery.

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