白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析

马河; 程艳琳; 张金杰; 曹广尚; 杨培民

doi:10.13422/j.cnki.syfjx.2014220037

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白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析

化学与分析 | 更新时间：2024-01-04

- 白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析
- Effect of Preliminary Immune Activity and Structural Identification of a Polysaccharide Extracted from
- 中国实验方剂学杂志 2014年20卷第22期页码：37-40
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81274052);山东省自然科学基金项目(ZR2011HL043);山东省优秀中青年科学家科研奖励基金项目 (BS2013YY052)
- DOI：10.13422/j.cnki.syfjx.2014220037
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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马河, 程艳琳, 张金杰, 等. 白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析[J]. 中国实验方剂学杂志, 2014,20(22):37-40.

MA He, CHENG Yan-lin, ZHANG Jin-jie, et al. Effect of Preliminary Immune Activity and Structural Identification of a Polysaccharide Extracted from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 37-40.
马河, 程艳琳, 张金杰, 等. 白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析[J]. 中国实验方剂学杂志, 2014,20(22):37-40. DOI： 10.13422/j.cnki.syfjx.2014220037.

MA He, CHENG Yan-lin, ZHANG Jin-jie, et al. Effect of Preliminary Immune Activity and Structural Identification of a Polysaccharide Extracted from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 37-40. DOI： 10.13422/j.cnki.syfjx.2014220037.

摘要

目的: 分离纯化白花蛇舌草总多糖

研究其对环磷酰胺致免疫低下小鼠的免疫增强活性.方法: 采用超滤技术分离纯化白花蛇舌草总多糖

高效凝胶渗透色谱-示差检测器方法测定相对分子质量

柱前衍生化HPLC分析其单糖组成;采用环磷酰胺致免疫低下小鼠碳粒廓清试验研究其免疫活性.结果: 从白花蛇舌草中分离得到1个单一多糖组分ODP-1

其相对分子质量20.88 kDa

单糖组成为甘露糖-鼠李糖-半乳糖醛酸-葡萄糖-半乳糖-阿拉伯糖

摩尔比0.005:0.033:0.575:1.000:0.144:0.143;免疫活性试验结果表明

ODP-1可升高环磷酰胺致免疫低下小鼠廓清指数、吞噬指数、胸腺指数和脾指数.结论: 白花蛇舌草多糖ODP-1为均一多糖

具有免疫增强活性.

Abstract

Objective:The polysaccharide was separated and purified from Oldenlandia diffusa and the immuno-enhancing activity was evaluated in immunosuppression mice by intraperitoneal administration. Method: The crude polysaccharides were separated and purified by tangential-flow ultrafiltration

then the relative molecular weight was measured by gel column chromatography. The structure was analyzed by pre-column derivatization HPLC. The activity of the homogeneous polysaccharide was investigated by carbon clearance on immunosuppression mice. Result: A new homogeneous polysaccharide named ODP-1 was obtained from O. diffusa. The relative molecular weight of ODP-1 was about 20.88 kDa. ODP-1 was composed of mannose

rhamnose

galacturonic acid

glucose

galactose and arabinose with the molar ratio of 0.005: 0.033: 0.575: 1.000: 0.144: 0.143.Immune activity indicated that ODP-1 can improve the clearance index

phagocytic index

the index of thymus and spleen of immunosuppression mice. Conclusion: ODP-1 from O. diffusa is a homogeneous polysaccharide with immuno-enhancing activity.

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