Objective:The aim of this paper was to study the HPLC fingerprint for oligosaccharides site in Rehmanniae Radix

and to provide reference for Rehmanniae Radix of the quality control according to the oligosaccharide composition. Method: Analysis was performed on an Agilent Zorbax NH2 column(4.6 mm×250 mm

5 μm)

with the acetonitrile-water as mobile phase in gradient elution mode. The flow rate was set at 1.0 mL ·min-1. The column temperature was maintained at 30 ℃. The injection volume was 20 μL. The detector was ELSD

drift tube temperature was set at 90 ℃

nitrogen flow rate was set at 3.0 L · min-1

and the gain volume was 10.Cluster analysis and principal component analysis were used to fingerprint pattern recognition. Result: Various peaks of thirteen batches of samples have been separated

and 12 peaks were identified as characteristic fingerprints in which six peaks were defined. And samples were divided into two categories. Conclusion: The established HPLC fingerprint can provide the basis for the quality control of Rehmanniae Radix.