HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量

樊化; 崔蕴慧; 利弈成; 王治平

doi:10.13422/j.cnki.syfjx.2014220049

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HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量
- Simultaneous Determination of Plantamajoside,Polydatin,Specnuezhenide and Resveratrol in Huzhentongfeng Capsule with HPLC
- 中国实验方剂学杂志 2014年20卷第22期页码：49-52
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"重大新药创制"科技重大专项(2011ZX09401-307);广东省科技厅项目(2012A080204003);广州市生物产业科技重大专项(201300000060);广东高校产学研结合示范基地项目(cgzhzd0905)
- DOI：10.13422/j.cnki.syfjx.2014220049
  中图分类号： O657.72;R286.0
- 纸质出版日期：2014
- 稿件说明：
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樊化, 崔蕴慧, 利弈成, 等. HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量[J]. 中国实验方剂学杂志, 2014,20(22):49-52.

FAN Hua, CUI Yun-hui, LI Yi-cheng, et al. Simultaneous Determination of Plantamajoside,Polydatin,Specnuezhenide and Resveratrol in Huzhentongfeng Capsule with HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 49-52.
樊化, 崔蕴慧, 利弈成, 等. HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量[J]. 中国实验方剂学杂志, 2014,20(22):49-52. DOI： 10.13422/j.cnki.syfjx.2014220049.

FAN Hua, CUI Yun-hui, LI Yi-cheng, et al. Simultaneous Determination of Plantamajoside,Polydatin,Specnuezhenide and Resveratrol in Huzhentongfeng Capsule with HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 49-52. DOI： 10.13422/j.cnki.syfjx.2014220049.

摘要

目的: 建立同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇含量的HPLC方法.方法: 采用高效液相法测定

色谱条件为Agilent SB-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%甲酸溶液梯度洗脱

检测波长280 nm

流速1.0 mL ·min-1

柱温30 ℃.结果: 大车前苷、虎杖苷、特女贞苷、白藜芦醇分别在0.099 6~1.992 (r=0.999 9)

0.123~2.46 (r=0.999 9)

0.145~2.90 (r=0.999 9)

0.025~0.50 μg(r=0.999 8)与峰面积呈良好的线性关系

平均回收率分别为99.27%

99.43%

98.83%

98.07%.结论: 该方法快速简便

重复性好

专属性强

可作为虎贞痛风胶囊的质量控制方法.

Abstract

Objective:The aim of this study was to establish a HPLC method for simultaneous determination of the contents of plantamajoside

polydatin

specnuezhenide and resveratrol in Huzhentongfeng capsules. Method: HPLC was used to determine the contents. The analytical column was Agilent SB-C18 column (4.6 mm×250 mm

5 μm). The liquid phase was composed of acetonitrile and 0.1% formic acid with gradient elution. The detection wavelength was set at 280 nm

the flow rate was 1.0 mL · min-1 and the column temperature was maintained at 30 ℃. Result: The mass of plantamajoside

polydatin

specnuezhenide and resveratrol with peak areas were within the concentration ranges of 0.099 6-1.992 μg(r=0.999 9)

0.123-2.46 μg(r=0.999 9)

0.145-2.90 μg(r=0.999 9)

0.025-0.50 μg(r=0.999 8)

and the average recoveries were 99.27%

99.43%

98.83%

98.07%

respectively. Conclusion: The method is rapid and simple

highly reproducible and specific so that it could be used as one of the quality control methods of Huzhentongfeng capsules.

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