枸杞子多糖柱前衍生HPLC指纹图谱分析

蒋梅; 谭丽蓉; 黄晓洁; 林伟斌; 吴小莉; 魏刚

doi:10.13422/j.cnki.syfjx.2014220053

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枸杞子多糖柱前衍生HPLC指纹图谱分析

化学与分析 | 更新时间：2024-01-04

- 枸杞子多糖柱前衍生HPLC指纹图谱分析
- Pre-column Derivatives HPLC Fingerprint of Lycii Fructus Polysaccharides
- 中国实验方剂学杂志 2014年20卷第22期页码：53-56
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省部产学研结合项目(2012B090600025)
- DOI：10.13422/j.cnki.syfjx.2014220053
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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蒋梅, 谭丽蓉, 黄晓洁, 等. 枸杞子多糖柱前衍生HPLC指纹图谱分析[J]. 中国实验方剂学杂志, 2014,20(22):53-56.

JIANG Mei, TAN Li-rong, HUANG Xiao-jie, et al. Pre-column Derivatives HPLC Fingerprint of Lycii Fructus Polysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 53-56.
蒋梅, 谭丽蓉, 黄晓洁, 等. 枸杞子多糖柱前衍生HPLC指纹图谱分析[J]. 中国实验方剂学杂志, 2014,20(22):53-56. DOI： 10.13422/j.cnki.syfjx.2014220053.

JIANG Mei, TAN Li-rong, HUANG Xiao-jie, et al. Pre-column Derivatives HPLC Fingerprint of Lycii Fructus Polysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 53-56. DOI： 10.13422/j.cnki.syfjx.2014220053.

摘要

目的: 建立枸杞子多糖柱前衍生HPLC指纹图谱.方法: 以三氟乙酸(TFA)水解枸杞子多糖

水解产物加入1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生化

采用HPLC测定枸杞子多糖中单糖的衍生物.采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相0.1 mol ·L-1磷酸缓冲液(pH 6.7)-乙腈(84.5:15.5)

流速0.8 mL ·min-1

检测波长250 nm

柱温30 ℃.结果: 枸杞子多糖柱前衍生指纹图谱标示出11个共有峰

鉴别了8个共有峰(D-甘露糖

L-鼠李糖

D-葡萄糖醛酸

D-半乳糖醛酸

D-葡萄糖

D-半乳糖

D-木糖

D-阿拉伯糖).10批枸杞子药材指纹图谱相似度>0.99.结论: 该方法简便

分离度高

重复性及稳定性良好

可有效控制枸杞子多糖的质量.

Abstract

Objective:The aim of this study was to establish a pre-column derivation HPLC method for chromatographic fingerprint of Lycii Fructus Polysaccharide. Method: Lycii Fructus sample was hydrolyzed with Trifluoroacetic Acid(TFA) and derivated by 1-phenyl-3-methyl-5-pyrazolone(PMP). The monosaccharide composition of Lycii Fructus polysaccharide was carried out by reversed-phase technique on a ZORBAX Eclipse XDB-C18 column with a mobile phase composed of 0.1 mol · L-1 phosphate buffer (pH 6.7) and acetonitrile in the ratio of 84.5: 15.5.The flow rate was set at 0.8 mL · min-1

the wavelength was set at 250 nm

and the column temperature was at 30 ℃. Result: There were 12 peaks from the fingerprint and 8 peaks were D-Man

L-Rha

D-GlcUA

D-GlcN

D-Glc

D-Gal

D-Xyl

D-Ara. The similarity of the 10 batches of Lycium barbarum samples was more than 0.99. Conclusion: The HPLC method with pre-column derivatization is appropriate for the analysis of monosaccharide composition of Lycii Fructus polysaccharide and the method is simple

quick and accurate.

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