HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量

安杉杉; 陈燕忠; 林世源; 陈卉; 方小爱; 吕竹芬

doi:10.13422/j.cnki.syfjx.2014220066

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HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量

化学与分析 | 更新时间：2024-01-04

- HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量
- Determination of Rosemary Acid and Luteolin in Defatted by HPLC
- 中国实验方剂学杂志 2014年20卷第22期页码：66-68
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- DOI：10.13422/j.cnki.syfjx.2014220066
  中图分类号： O657.72;R284.1
- 纸质出版日期：2014
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安杉杉, 陈燕忠, 林世源, 等. HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量[J]. 中国实验方剂学杂志, 2014,20(22):66-68.

AN Shan-shan, CHEN Yan-zhong, LIN Shi-yuan, et al. Determination of Rosemary Acid and Luteolin in Defatted by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 66-68.
安杉杉, 陈燕忠, 林世源, 等. HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量[J]. 中国实验方剂学杂志, 2014,20(22):66-68. DOI： 10.13422/j.cnki.syfjx.2014220066.

AN Shan-shan, CHEN Yan-zhong, LIN Shi-yuan, et al. Determination of Rosemary Acid and Luteolin in Defatted by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 66-68. DOI： 10.13422/j.cnki.syfjx.2014220066.

摘要

目的: 建立HPLC同时测定脱脂紫苏子中迷迭香酸和木犀草素含量的方法.方法: 采用Diamonsil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%磷酸溶液(55:45)

流速1 mL ·min-1

检测波长330

350 nm

柱温30 ℃.结果: 迷迭香酸在9.91~158.55 mg ·L-1回归方程为Y=108.83X+0.14 (r=0.999 9)

平均回收率为99.68%

RSD 2.01%;木犀草素在0.997~15.952 mg ·L-1回归方程为Y=119.63X-3.68(r=0.999 9)

平均回收率为100.34%

RSD 2.86%.结果: 该方法操作简便、准确

重复性良好

可用于测定脱脂紫苏子中迷迭香酸和木犀草素的含量.

Abstract

Objective:The aim of this study was to establish an HPLC method for simultaneous determination of the contents of Rosemary acid and Luteolin in defatted Perilla. Method: Diamonsil C18 (4.6 mm×250 mm

5 μm) column was used

with methanol-0.1% phosphoric acid(55: 45) as mobile phase. The flow rate was set at 1.0 mL · min-1

the detection wavelength was set at 330 and 350 nm

with the column temperature at 30 ℃. Result: The linear response ranges were 9.91-158.55 mg · L-1 with regression curve Y=108.83X+0.136 8 (r=0.999 9) for rosemary acid

and 0.997-15.952 mg ·L-1 with regression curve Y=119.63X-3.677 8(r=0.999 9) for luteolin. The average recovery of rosemary acid and luteolin were 99.68% and 100.34%

respectively (RSD 2.01%

2.86%). Conclusion: A simple

accurate and reproducible HPLC method has been provided for simultaneous determination of rosemary acid and luteolin in defatted Perilla.

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