Objective:To establish an HPLC method for simultaneous determination of benzoylmesaconine

benzoylaconine

benzoylhypacoitine and hypaconitine in Wuqi Qufengsan tablets. Method: The assay was performed on a Hanbon-C18(4.6 mm×250 mm

5 μm)

mobile phase consisted of acetonitrile-tetrahydrofuran (25: 15

phase A)-0.1 mol · L-1 ammonium acetate(containing 0.5 mL glacial acetic acid per 1 000 mL

phase B) with gradient elution at the flow rate of 1.0 mL · min-1

column temperature was 30 ℃

and the detection wavelength was at 235 nm. Result: Excellent chromatographic separation was achieved for four studied alkaloids with good linearity(r>0.999 0)

the average recoveries were 101.10%(RSD 2.1%)

101.09%(RSD 2.2%)

102.04%(RSD 1.6%)

97.83%(RSD 2.7%). The limits of detection were 3.4

1.7

2.2and 0.8 ng respectively. Conclusion: This method was simple

accurate and reproducible

it can be used to determine the content of benzoylmesaconine

benzoylaconine

benzoylhypacoitine and hypaconitine in Wuqi Qufengsan tablets.