Objective: To establish a method of fingerprint analysis on Brucea Javanica oil latex Injection by HPLC-ELSD and provide a reference for the quality evalation of Brucea Javanica oil latex Injection. Method: The sample were determined by shimadzu 2010C HPLC and Alltech 2000 ESLD with Waters C18 analytical column (4.6 mm×250 mm

5μm)

by using acetonitrile-dichloromethane(65: 35) as the mobile phase at the flow rate of 1.0 mL · min-1 and the detection temperature of 70 ℃

column temperature 30 ℃

inject volume 10 μL. Result: Its fingerprint exhibited better separation for each peak

revealing amount of finger information

based on the ten batches of Brucea Javanica oil latex Injection

eight common peaks were selected in chromatograms. The precision reproducibility and stability of the method is good

and RSD of the relative retention time and relative peak area were less than 3.0%

the similarity is greater than 0.99

according to the technical requirements of fingerprint on Chinese tradirional medicine . Conclusion: Proposed HPLC fingerprint is convenient

reliable and can be used as the important evidences for the quality control of Brucea Javanica oil latex Injection.