Objective:To investigate the chemical constituents in seed of Sophora alopecuroides and to determine their antitumor activities in vitro. Method: Compounds were isolated and purified by means of silica gel and Sephadex LH-20 gel columm chromatography

and recrystallization etc. Their structures were elucidated using spectroscopic analysis including 1H-NMR

13C-NMR and spectral data. The cytotoxic activities of the alkaloids compounds on C6 cell in vitro were evaluated by MTT method. Result: Seven compounds were isolated from the ethanol extract of the seed of S. alopecuroides. Their structrues were identified as (+)-matrine(1)

(+)-oxymatrine(2)

(-)-sophocarpine(3)

(-)-9α-hydroxysophocarpine(4)

(+)-oxysophocarpine(5)

(-)-9α-hydroxysophoramine(6)

(-)-13

14-dehydrosophoridine(7)

and a non-alkaloid named indolyl-3-carbaldehyde(8). Conclusion: The compond 8 was isolated from the genus sophora for the first time. Compounds 1-3 and 5 showed significant inhibitory activity towards C6 glioma cells.