HPLC同时测定牛皮杜鹃中3种成分的含量及其提取方法的比较

关颖丽; 何晓燕; 姚慧敏

doi:10.13422/j.cnki.syfjx.2014220100

您当前的位置：

首页 >

文章列表页 >

HPLC同时测定牛皮杜鹃中3种成分的含量及其提取方法的比较

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定牛皮杜鹃中3种成分的含量及其提取方法的比较
- Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method
- 中国实验方剂学杂志 2014年20卷第22期页码：100-103
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2012]第360号)
- DOI：10.13422/j.cnki.syfjx.2014220100
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
关颖丽, 何晓燕, 姚慧敏. HPLC同时测定牛皮杜鹃中3种成分的含量及其提取方法的比较[J]. 中国实验方剂学杂志, 2014,20(22):100-103.

GUAN Ying-li, HE Xiao-yan, YAO Hui-min. Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 100-103.
关颖丽, 何晓燕, 姚慧敏. HPLC同时测定牛皮杜鹃中3种成分的含量及其提取方法的比较[J]. 中国实验方剂学杂志, 2014,20(22):100-103. DOI： 10.13422/j.cnki.syfjx.2014220100.

GUAN Ying-li, HE Xiao-yan, YAO Hui-min. Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 100-103. DOI： 10.13422/j.cnki.syfjx.2014220100.

摘要

目的: 建立HPLC同时测定长白山牛皮杜鹃中芦丁、槲皮素及山柰酚含量的方法

并比较不同提取方法对其含量的影响.方法: 采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm

5 μm);乙腈-0.1%磷酸水溶液进行梯度洗脱;检测波长为360 nm

流速为0.8 mL ·min-1

柱温为30 ℃.结果: 芦丁、槲皮素、山柰酚的在一定范围内线性关系均良好;平均加样回收率分别为99.9%(RSD 1.2%)

99.2%(RSD 2.8%)

101.8%(RSD 2.3%).结论: 该方法简便、快速、准确可靠.3种不同提取方法中以超声提取法所得黄酮类含量均最高.可用于长白山牛皮杜鹃的质量控制.

Abstract

To establish an HPLC method for the simultaneous determination of rutin

quercetin and populnetin in Rhododendron chrysanthum from Changbai mountain and compare their contents by different extractive methods. The HPLC equipped with the Agilent Eclipse XDB-C18column (4.6 mm×250 mm

5 μm). The mobile phase consisted of acetonitrile and 0.1% phosphoric acid with gradient elution. The detection wave length was set at 360 nm and the flow rate was 0.8 mL · min-1

and the temperature was set at 30 ℃. The linear ranges of rutin

quercetin and populnetin were good

the average recoveries were 99.9%(RSD 1.2%)

99.2%(RSD 2.8%)

101.8%(RSD 2.3%)

respectively. The method is simple

quick

accurate and repeatable for quantitative analysis. The supersonic extraction showed the highest content of flavonoids among the three extractive methods. The method can be used for quality control of R. chrysanthum from Changbai mountain.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

薄层色谱扫描法测定槐米中的芦丁和槲皮素

苦荞提取物中芦丁和槲皮素的含量测定

反相高效液相色谱法测定新疆枸杞叶茶中芦丁和槲皮素的含量

芦丁分解速度与加热温度的相关性