Objective:Through studying time change rule of compositions derived from Coptidis Rhizoma absorbed into the blood

to clear in vivo directly effective substance and metabolic process of Coptidis Rhizoma. Method: Serum pharmacochemistry method was adopted to study distribution

number and state(prototype components or metabolites) of chemical compositions from Coptidis Rhizoma absorbed into the body

then investigate its variation with time. Result: This study determined five components(epiberberine

jateorhizine

coptisine

palmatine and berberine hydrochloride) among these nine components from Coptidis Rhizoma absorbed into the body;in addition to peak 2 identified as metabolites by analysis

other peaks were in prototype form into the body.After peak 3

4

5

6

7

8

9 were absorbed into the blood

blood drug concentration reached its peak in a certain period of time(120 min)

peak time was longer

and then was eliminated with the blood circulation in the body.After peak 3 and 4 were absorbed into the blood

blood drug concentration decreased

but their relative contents were increased

which indicated conversion of other components to them. Conclusion: Main components of compositions derived from Coptidis Rhizoma absorbed into the blood can be detected at 60 min after administration

they reach peak value at 120 min

their contents at 240 min are similar to these at 60 min;minor components can be transformed from other components.Effective components directly in the body may be determined by analyzing components of drugs from serum after taking medicines orally

scientific administration and combination scheme of traditional Chinese medicine can establish by according to change rule of components from serum after taking medicines orally.