Objective:To investigate the anti-aging effect of ethanol extract of Fraxini Cortex (EEFC)in subacute aging mice. Method: Sixty ICR mice were randomly divided into the blank group (physiological saline 100 mg · kg-1)

the model group (D-galactose 100 mg · kg-1)

the positive group(D-galactose 100 mg ·kg-1+vitamin E 30 mg · kg-1)

the low dose group D-galactose 100 mg · kg-1+EEFC 37.5 mg · kg-1)

the dose group (D-galactose 100 mg · kg-1+EEFC 75 mg · kg-1)

the high dose group(D-galactose 100 mg · kg-1+EEFC 150 mg · kg-1)

each group of 10.And mice were treated by intragastric administration for 60 days

at the last day of performing intragastric administration

taking out the blood from eyeball

then took out the brains

thymus

spleens

livers and skin

and measure the viscera index of brains

thymus and spleens.Finally detected the superoxide dismutase (SOD)

catalase(CAT)

glutathione peroxidase(GSH-Px)

malondialdehyde(MDA)

total antioxidant capacity(T-AOC). Result: Compared with the blank group group

the model group the viscera index of brains

thymus and spleens decreased obviously(P<0.05

P<0.01)

T-AOC

SOD

CAT in serum

livers

brains and skin decreased

and MDA increased. The viscera index of brains

thymus and spleens have increased a lot(P<0.05

P<0.01) after treateded EEFC. All dosage groups of EEFC could increase the activity of SOD

CAT in serum

livers

brains and thymus(P<0.05

P<0.01)

and reduce the content of MDA in these organizations(P<0.05

P<0.01)

meanwhile the T-AOC was increased obviously(P<0.05

P<0.01)

and increase the activity of GSH-Px in serum and skin. Conclusion: EEFC has anti-aging effect on the subacute aging model mice which were Established by the injection of D-galactose.