Objective:To study the effect of Jiawei Danggui Buxue Tang(JWDGBXT) on daunomycin-induced nephropathy (DN) by in rats. Method: SD rats were divided into four groups

including normal controls

model group

benazeprilgroup(1 mg · kg-1) JWDGBXT(10 g · kg-1). The adriamycin atdose of 6 mg · kg-1 was used to establish the model. The benepril or JWDGBXT was given by intragastric administration. 7

28

42 and 56 days after the treatment

the levels of microdosis proteinuria were tested. The renal tissue was processed for the examinations of light microscopy and immunohistochemistry. Then the renal tissue was assayed by the way of RT-PCR and Wetern blot to observe the level of nephrin

nestin and vimentin. Result: Compared with the normal group

model group had obvilus changes at the 24 h urine protein(P<0.05). Moreover

JWDGBXT could significantly reduce protein uria in rats with daunomycin-induce nephropathy than model group and benepril group(P<0.05). The intercapillary cells had different hyperplasy of the SD rats. The pathological changes of renal tissue at renal tubular and renal interstitial were analyzed. Then

the level of nephrin

nestin and vimentin of the renal tissue was detected by the way of RT-PCR and Western blot. The level of nephrin

nestin and vimentin wa improved. Conclusion: JWDGBXT could decrease the level of proteinuria

protecte the podocytes and suppress the hyperplasy of the intercapillary cells. All of the actions may be associated with the cytoskeletal protein:nestin and vimentin.