Objective:To investigate the effects of Eucommia ulmoides flavonoids astragalin and baicalein on the expression of osteoblast specific transcription factor Osterix and the ratio of osteoproteger and receptor activator of nuclear factor kappa B ligand (OPG/RANKL). Method: MC3T3-E1 Subclone 14 osteoblasts were cultured with different concentrations of astragalin

baicalein and vitamin D3 for 24 h. MTT detection method was used to detect cell proliferati. The expression of Ca2+ was detected by ELISA. The expressions of OPG

RANKL

OST were detected by Western blotting. Result: Compared with the control group

astragalin and baicalein can significantly promote the proliferation of MC3T3-E1 Subclone 14 (P<0.05)

increase the expression of OPG and Osterix obviously (P<0.05)

and reduce the expression of RANKL (P<0.05). Conclusion: It shows that astragalin and baicalein from E. ulmoides flavonoids can promote MC3T3 E1 Subclone 14 osteoblast proliferation

and raised Osterix and OPG/RANKL.