杜仲药材有效成分与环境因子的灰色关联度分析

蔡萍; 刘才英; 梁雪娟; 肖娟; 万丹; 张水寒

doi:10.13422/j.cnki.syfjx.2014230010

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杜仲药材有效成分与环境因子的灰色关联度分析

工艺与制剂 | 更新时间：2024-01-04

- 杜仲药材有效成分与环境因子的灰色关联度分析
- Grey Correlation Degree Analysis Between Active Ingredients in Barks or Leaves of Eucommiae Cortex and Environmental Factors in Their Habitats
- 中国实验方剂学杂志 2014年20卷第23期页码：10-14
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目(2011BAI01B08)
- DOI：10.13422/j.cnki.syfjx.2014230010
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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蔡萍, 刘才英, 梁雪娟, 等. 杜仲药材有效成分与环境因子的灰色关联度分析[J]. 中国实验方剂学杂志, 2014,20(23):10-14.

CAI Ping, LIU Cai-ying, LIANG Xue-juan, et al. Grey Correlation Degree Analysis Between Active Ingredients in Barks or Leaves of Eucommiae Cortex and Environmental Factors in Their Habitats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 10-14.
蔡萍, 刘才英, 梁雪娟, 等. 杜仲药材有效成分与环境因子的灰色关联度分析[J]. 中国实验方剂学杂志, 2014,20(23):10-14. DOI： 10.13422/j.cnki.syfjx.2014230010.

CAI Ping, LIU Cai-ying, LIANG Xue-juan, et al. Grey Correlation Degree Analysis Between Active Ingredients in Barks or Leaves of Eucommiae Cortex and Environmental Factors in Their Habitats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 10-14. DOI： 10.13422/j.cnki.syfjx.2014230010.

摘要

目的: 运用灰色关联度法分析影响杜仲皮和叶中4种有效成分含量产地差异性的主要环境因子. 方法: 利用HPLC同时测定10个产地10年生杜仲皮和叶中桃叶珊瑚苷、京尼平苷酸、绿原酸、松脂醇二葡萄糖苷的含量

流动相分别为乙腈(A)-0.1%磷酸水(B)梯度洗脱(0~25 min

5%~13%A;25~45 min

13%~15%A;45~80 min

15%~21%A)

流动相乙腈(A)-0.1%磷酸水(B)梯度洗脱(0~20 min

5%~12%A;20~35 min

12%~13%A;35~45 min

13%~19%A;45~65 min

19%~25%A)

检测波长均为210 nm.检测杜仲生长地土壤的全氮、全磷、全钾、碱解氮、速效磷、速效钾、有机质及pH

获得土壤因子数据

运用灰色关联度法对4种有效成分与气候及土壤因子的相关性进行分析. 结果: 10个产地同一生长年限杜仲皮、叶中4种有效成分的含量存在较大差异.碱解氮、速效钾、有机质、年平均相对湿度、年平均最高气温对4种成分在杜仲不同组织中的影响较大. 结论: 杜仲不同组织部位中4种有效成分含量与环境因子的灰色关联度分析明确了主导环境因子

为合理施肥及环境调控来提高杜仲次生代谢产物的含量提供参考

为杜仲的适宜种植区选择及其道地性产生原因分析提供实验依据.

Abstract

Objective: To find out dominant environmental factors which impact main effective components in barks or leaves of Eucommiae Cortex by gray correlation analysis. Method: HPLC was employed to determine contents of geniposidic acid

aucubin

pinoresinol diglucoside and chlorogenic acid at the same time in barks or leaves of Eucommiae Cortex at 10 years from ten habitats

detection wavelength were 210 nm.Soil physicochemical characteristics were detected

such as total nitrogen

total phosphorus

total potassium and so on

in order to obtain soil factor data

data were analyzed by grey correlation method. Result: There were big differences between barks or leaves of Eucommiae Cortex at the same growth ages in ten habitats.Effects of alkali-hydrolyzable nitrogen

rapidly available potassium

organic matter

the annual average relative humidity

the annual average maximum temperature on contents of four compounds from different tissue were remarkable. Conclusion: Grey correlation analysis finds out dominant environmental factors that impact contents of active ingredients in different tissues of Eucommiae Cortex.It may provide a clue to improve content of secondary metabolites in Eucommiae Cortex by rational application of fertilizer and environmental control

it also provides scientific guidance for choosing suitable areas and analyzing genuineness causes of Eucommiae Cortex.

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