Objective:To optimize enzymatic extraction process of Cuscutae Semen

in order to increase extracting rates of hyperin and total flavonoids. Method: With the content of hyperin as index

effects of enzyme species

enzyme dosage

enzymolysis time

pH and temperature on enzymatic extraction process were investigated by single factor test.With composite score of extracting amounts of hyperin and total flavonoids as index

orthogonal test was used to optimize enzymatic extraction process with enzyme dosage

enzymolysis pH and temperature as factors.HPLC was employed to determine the content of hyperin with mobile phase of acetonitrile-0.1% phosphoric acid solution(17: 83) and detection wavelength at 360 nm. Result: Cellulase was selected

its optimum extraction technology was as follows:enzyme dosage 2.0%

enzymolysis time of 2 hours

enzymatic hydrolysis of pH 4.0

enzymolysis temperature at 45 ℃;extracting amounts of hyperin and total flavonoids were 1.408

26.966 mg · g-1

respectively. Conclusion: This optimized extracting process is stable

reasonable and applicable to industrial production of Cuscutae Semen.