GUO Xiao-mei, HE Wen-bin, LIU Guang-tao, et al. HPLC Fingerprint Analysis of Lancong Tea[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 71-74.
GUO Xiao-mei, HE Wen-bin, LIU Guang-tao, et al. HPLC Fingerprint Analysis of Lancong Tea[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 71-74. DOI: 10.13422/j.cnki.syfjx.2014230071.
Objective: To establish the HPLC fingerprint of Lancong tea. Method: HPLC was adopted in the fingerprint analysis
and the chromatographic conditions were as follows:InertsilRRSOD-C18(4.6 mm×250 mm
5 μm) column;mobile phase consisted of methanol(A)-0.1% phosphorous acid(B) solution for gradient elution 0-15 min
5%-15%A;15-43 min
15%-45%A;43-55 min
45%-62%A;55-80 min
62%A. The detection wavelength of 273 nm;the column temperature of 20℃.The flow rate of 1.0 mL· min-1
gallic acid peak as the reference peak. Result: There were mainly 15 characteristic peaks in the fingerprints. Among these peaks
13 peaks belonged to Canarii Fructus
2 peaks belonged to Perillae Folium
and the 2nd one was gallic acid peak.The similarity of HPLC fingerprints of 10 batches of Lancong tea were more than 0.99. Conclusion: The fingerprint is stable and feasible
and can be used to control the quality of Lancong tea.