Objective: To determine the contents of cucurbitacins B in some parts of Trichosanthes kirilowii Maxim. Method The sample solutions were prepared in 50% MeOH by the supersonic extraction. An HPLC-UV method was performed on ODS column (4.6 mm×250 mm

5 μm) with a mobile phase of methanol-water(70: 30) at a flow rate of 1.0 mL·min-1

the detection wavelength was at 230 nm

and column temperature at 30℃. Result Cucurbitacins B and E were determined in male root

female root

stem

leaf

young fruit

unripe fruit

ripe fruit

unripe flesh

ripe flesh

seed

male flower

carpopodium

unripe pericarp

and ripe pericarp. The contents of cucurbitacin B were 0-23.13 mg per 100 g sample of each part of T. kirilowii. Cucurbitacin E was not detected in any parts of T. kirilowii. Conclusion: There are different contents of cucurbitacin B in different parts of T. kirilowii. The content of cucurbitacin B was 23.13 mg per 100 g of a stouk root of female T. kirilowii growth for 3 years and lower than 9 mg per 100 g of other samples. The contents of cucurbitacin B were related to some factors such as the growth period and the part of T. kirilowii.