Objective: To study the chemical constituents of Paeonia mairei in Taibai mountains and to supply material basis for its pharmacological research and therapeutic mechanism. Method: The compounds from P. mairei were isolated and purified by silica gel column chromatography

Sephadex LH-20 column chromatography and recrystallization method

and their structures were elucidated on the basis of their physicochemical properties and spectra analysis. Result: Eight compounds were obtained and identified as β-sitosterol(1)

oleanic acid(2)

hederagenin(3)

daucosterol(4)

chrysophanol(5)

benzoic acid(6)

gallic acid(7)

ethyl gallate(8). Conclusion: Compound 5 were obtained from the genus for the first time. The rest of the compounds was isolated from the plant of P. mairei for the first time.