翻白草总黄酮对2型糖尿病大鼠胰岛素底物-2-磷脂酰肌醇-3激酶信号通路的影响

胡建新; 周志愉; 王晓敏; 邹志坚; 席晓甜

doi:10.13422/j.cnki.syfjx.2014230146

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翻白草总黄酮对2型糖尿病大鼠胰岛素底物-2-磷脂酰肌醇-3激酶信号通路的影响

药理 | 更新时间：2024-01-04

- 翻白草总黄酮对2型糖尿病大鼠胰岛素底物-2-磷脂酰肌醇-3激酶信号通路的影响
- Effect of Flavonoids from on Insulin Substrate -2-Phosphatidylinositol-3 Kinase Signaling Pathway on Type 2 Diabetes Mellitus Rat
- 中国实验方剂学杂志 2014年20卷第23期页码：146-150
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省卫生厅科技项目(20131268);江西省卫生厅中医药科技项目(2013A022);江西中医药大学课题(2013ZR0048)
- DOI：10.13422/j.cnki.syfjx.2014230146
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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胡建新, 周志愉, 王晓敏, 等. 翻白草总黄酮对2型糖尿病大鼠胰岛素底物-2-磷脂酰肌醇-3激酶信号通路的影响[J]. 中国实验方剂学杂志, 2014,20(23):146-150.

HU Jian-xin, ZHOU Zhi-yu, WANG Xiao-min, et al. Effect of Flavonoids from on Insulin Substrate -2-Phosphatidylinositol-3 Kinase Signaling Pathway on Type 2 Diabetes Mellitus Rat[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 146-150.
胡建新, 周志愉, 王晓敏, 等. 翻白草总黄酮对2型糖尿病大鼠胰岛素底物-2-磷脂酰肌醇-3激酶信号通路的影响[J]. 中国实验方剂学杂志, 2014,20(23):146-150. DOI： 10.13422/j.cnki.syfjx.2014230146.

HU Jian-xin, ZHOU Zhi-yu, WANG Xiao-min, et al. Effect of Flavonoids from on Insulin Substrate -2-Phosphatidylinositol-3 Kinase Signaling Pathway on Type 2 Diabetes Mellitus Rat[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 146-150. DOI： 10.13422/j.cnki.syfjx.2014230146.

摘要

目的: 研究翻白草总黄酮对2型糖尿病大鼠糖脂代谢和肝组织胰岛素底物-2-磷脂酰肌醇-3激酶(IRS-2-PI3-K)信号通路的影响. 方法: 翻白草经溶剂提取

聚酰胺柱分离提取得总黄酮提取物;以高脂高糖乳剂加链脲佐菌素复制2型糖尿病大鼠模型

将造模成功的大鼠随机分为翻白草总黄酮高剂量(216 mg·kg﹣1)和低剂量(108 mg·kg﹣1)、模型对照组、阳性药组(盐酸二甲双胍悬浊液20.8 mg·kg-1)

连续给药4周后

观察其血糖、血脂、游离脂肪酸、肝糖原、胰岛素水平的变化

采用Western blots法检测肝组织IRS-2和PI-3K蛋白的表达. 结果: 与正常组比较

模型组大鼠体重下降、血糖及总胆固醇(TC)升高;高密度脂蛋白胆固醇(HDL-C)下降(P<0.05).与模型组比较

翻白草总黄酮高剂量组血糖下降(P<0.05)

TC下降

HDL-C升高(P<0.05);胰岛素和胰岛素抵抗指数均降低(P<0.05);肝组织IRS-2和PI-3K蛋白的表达量升高(P<0.05). 结论: 翻白草总黄酮能纠正2型糖尿病大鼠糖代谢紊乱

可能是通过降低减少肝糖原的输出

提高肝组织胰岛素IRS-2-PI3-K蛋白表达

从而减弱外周胰岛素抵抗.

Abstract

Objective: To observe the effects of flavonoids from Potentilla discolor (FPD) on insulin substrate-2-phosphatidylinositol-3kinase(IRS-2-PI3K)signaling pathway of liver tissue on type 2 diabetes mellitusmellitus (T2DM) rat. Method: Male Sprague-Dawley(SD) rats were administrated with enriched diet and injected with streptozotocin(STZ

30 mg· kg-1)to make the model of rat T2DM. The changes of serum fasting blood glucose (FBG)

blood lipids

serum fasting insulin (FINS)

liver glycogen and free fatty acid were observed. The expressions of IRS-2

PI3-K in liver tissue were determined with Western blots. Result: Compared with normal group

FBG and total cholesterin(TC)were increased

while body weight and high-density lipoprotein cholesterol (HDL-C)were decreased (P<0.05).Compared with model group

FBG

FINS and insulin resistance index in T2DM rats treated with FPDB were decreased significantly(P<0.05).TC were decreased and HDL-C were increased (P<0.05).IRS-2 and PI3-K protein expressions were obviously increased (P<0.05). Conclusion: The effect of FPDB in treating T2DM was probably contributed to adjusting the dyperfunction of glucose and lipid metabolism and associated with its improvement IRS-2--PI3K protein expressions of hepatic insulin on T2DM rat.

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