Objective: To observe the effect of Qutan Huoxue decocotion (QTHXD) on the expression of aquaporin 9 (AQP9) in the nonalcoholic fatty liver rats

and discuss the mechanism. Method: Sixty SD rats were randomly divided into 5 groups

12 each group:the blank control group

model control group

QTHXD low

medium and high dose groups(6

12

16 g· kg-1)

all rats except balnk congtrol group were fed high fat diet for 10 weeks to establish nonalcoholic fatty liver disease(NAFLD) model. After modelingg

the corresponding drugs were ig giventwice a day

for 2 weeks.HE

ELISA and RT-PCR were used to observe the liver pathology changes

total cholesterol(TC)

triglycerides(TG) and the AQP9 mRNA expression in liver cells. Result: ① AQP9 expression in liver cells of NAFLD model group compared with blank control group was raised.

but decreased in three QTHXD dose 12 g· kg-1group was lower than the other two groups.②TC and TG in three QTHXD dose groups deacreased

16 g· kg-1group was lower than the other two groups.③Hepatic steatosis in the QTHXD different dose groups was reduced compared with NAFLD model group

and hepatic steatosis in 16 g· kg-1 group reduced more markedly than the other two groups. Conclusion: QTHXD could regulate AQP9 expression of liver cells in NAFLD model rats

reduce TC and TG and improve the degree of hepatic steatosis.