Objective: To study the effect of Ailuo Kechuanning on expression of p38MAPK

tumor necrosis factor alpha(TNF-α)

and aquaporin 5(AQP5) genes in rats with chronic obstructive pulmonary disease(COPD). Method: Lipopolysaccharide(LPS)smoke was used to COPD rat model

the experimental animals were randomly divided into normal control group

model group

Normal group

model group was given physiological saline(15.52 mL· kg-1· d-1) byintragastric

Ailuo Kechuanning group(7.75

15.52

31.04 g· kg-1· d-1)

ig for 15 days. The expression of p38MAPK

TNF-α and AQP5 genes in the trachea

bronchus and lung tissue were detected by in situ hybridization and immunohistochemical techniques. Result: Compared with the normal group

the expression of mRNA and protein of AQP5 were weakened

but the expression of p38MAPK

TNF-α mRNA and protein were enhanced in model group

the expression of AQP5 protein was negatively correlated with p38MAPK(r=-0.547

P<0.01)

TNF-α protein(r=-0.532

P<0.01).Compared Ailuo Kechuanning mediate-dose group with the model group

the expression of AQP5 gene in enhancements

However

the expression of p38MAPK

TNF-α genes was decreased apparently

the expression of AQP5 protein was negatively correlated with p38MAPK(r=-0.542

P<0.01)

TNF-α protein(r=-0.541

P<0.01). Conclusion: The mechanism of Ailuo Kechuanning regulating COPD airway mucus hypersecretion may increase AQP5 gene and inhibit p38 MAPK

TNF-α genes expression.