Objective: To study the effect of Banxia Xiexin Tang on intestinal immune function in fluorouracil-induced diarrhea mice

and to reveal the therapeutic mechanism on chemotherapy-induced diarrhea. Method: The fluorouracil(40 mg· kg-1) was intraperitoneal injected for 6 days to establish the diarrhea mice model. Sixty ICR mice were randomized into 6 groups:normal group

model group

montmorillonite powder group(1.17 g·kg-1)

Banxia Xiexin Tang(2.5

1.25

0.625 g· kg-1) group

administrated by oral gavage for 2 day before modeling

the intervention period lasted 11 days. Then we observed the changes of daily activity status

food intake

body weight

diarrhea

measured the quality of the thymus and spleen

observed the pathological changes of ileal tissue

measured the expression of intestinal immunoglobulin A (IgA)

vasoactive intestinal peptide (VIP)

interleukin-15(IL-15)by ELISA method. Result: Compared to normal group

the diarrhea of model group mice was aggravated and the weight

thymus and spleen index were decreased

the levels of IgA and VIP were decreased

whereas the IL-15 was increased.Compared to model group

Banxia Xiexin Tang could significantly inhibit body weight loss

diarrhea and spleen

thymus reduction induced by fluorouracil in mice. Banxia Xiexin Tang could increase the levels of IgA and VIP and decrease IL-15 expression (P<0.05

P<0.01). Conclusion: Banxia Xiexin Tang could prevent the fluorouracil induced diarrhea by up-regulating the expression of IgA

VIP and down-regulating the expression of IL-15

thereby generating immune protection.