Objective: To observe effects of Jiawei Duhuo Jisheng Tang on mRNA transcription expression levels of interleukin-17(IL-17)

tumor necrosis factor-α(TNF-α)

interleukin-6(IL-6) in knee joint synovial and protein expression in serum of type Ⅱ collagen-induced arthritis in rats. Method: A rat model of collagen induced arthritis(CIA) was developed with male SPF sprague-dawley rats

thirty CIA rats were divided randomly into 5 groups

the model group were given distilled water(ig

10 mL· kg-1)

the leflunomide group(ig

10 mL· kg-1)

low

medium and high dosage groups of Jiawei Duhuo Jisheng Tang(ig

11

22

44 g· kg-1)

6 rats each group.Rats in the normal group were given the same dosage of distilled water

all rats received oral administration of corresponding medicines once daily for 12 weeks.Levels of interleukin-6(IL-6)

interleukin-17(IL-17) and tumor necrosis factor-α(TNF-α) in serum were examined by ELISA

mRNA transcription expression levels of IL-6

IL-17

TNF-α were detected by RT-PCR.Statistical methods was employed by variance analysis. Result: Compared with the normal group

mRNA and protein expression of IL-6

IL-17

TNF-α increased significantly in the model group. Compared with the model group

mRNA and protein of IL-6

TNF-α in treatment groups was down-regulated

protein expression of IL-17 of Jiawei Duhuo Jisheng Tang decreased significantly and mRNA expression of IL-17 was no significant differences. Conclusion: Jiawei Duhuo Jisheng Tang shows a certain therapeutic effect on CIA rats

its anti-inflammatory action mechanism may achieve by reducing expression of IL-6

IL-17 and TNF-α.