Objective: To optimize extraction process of Mori Folium leaves in Yinsang Jiangtang granules. Method: Taking extracting amount of rutin as index

L9(34) orthogonal design was adopted to optimize alcohol extraction technology with extraction time

ethanol amount and concentration as factors.With relative yield of 1-deoxynojirimycin(DNJ) as index

L9(33) orthogonal design was used to optimize water extraction technology by taking solid-liquid ratio and extraction time as factor.The content of rutin was determined by HPLC

detection wavelength was 358 nm

mobile phase consisted of methanol(A)-0.5% phosphoric acid solution(B) for gradient elution(0-5 min

30%A;5-10 min

30%-35%A;10-15 min

35%-40%A;15-18 min

40%-50%A).The contents of DNJ were detected by precolumn derivation HPLC-UV method with FMOC-Cl as reagent. Result: Optimum alcohol extraction press was as following:extracted twice with 20 times the amount of 60% ethanol for 1.5 h;extracting amount of rutin was 1.22 mg·g-1.Optimum water extraction process was extracted twice for 1.0

0.5 h with 30

25 times the amount of water;relative yield of DNJ was 88.06%. Conclusion: These optimized extraction technologies are stable and feasible

it can balance extraction of flavonoids and alkaloids in Mori Folium.