Objective: To optimize degreasing technology of extracts from Selaginella moelledorfii with petroleum ether

which provides a reference to development and utilization of the plant resources. Method: Taking extraction amount of amentoflavone and yield of extract as evaluation indexes

effects of the amount of petroleum ether

extraction time and frequency on degreasing technology were investigated by orthogonal design.The content of amentoflavone was determined by HPLC

mobile phase consisted of acetonitrile(A)-water(B) for gradient elution(0-5 min

25%A;5-12 min

25%-35%A;12-17 min

35%-45%A;17-25 min

45%-50%A;25-40 min

50%-55%A;40-45 min

55%-70%A;45-50 min

70%-100%A;50-55 min

100%A;55-60 min

100%-25%A) and detection wavelength at 330 nm. Result: Optimum technology conditions were as follows:degreased twice with 6 times the amount of petroleum ether for 1 h per time;extraction amount of amentoflavone was 72.77 mg

yield of extract was 11.04%. Conclusion: This degreasing technology is rational and stable

which is available for purification of extracts from S. moelledorfii.