Objective: Stability of preparation process of total flavonoids and total saponins from Ziziphi Spinosae Semen was investigated

in order to provide a guarantee for sample preparation of follow-pharmacodynamic evaluation. Method: Ten batches of total flavonoids and total saponins from Ziziphi Spinosae Semen were prepared with the same preparation process.Spinosin

betulinic acid

jujuboside A and jujuboside B were taken as index components

ELSD/HPLC was employed to analyze feature profiles of these two parts samples with mobile phase of acetonitrile(A)-water(B) for gradient elution(0-10 min

12%-18%A;11-20 min

19%-23%A;21-30 min

23%-34%A;31-40 min

35%-45%A;41-50 min

46%-100%A;51-60 min

100%-11%A;61-70 min

12%A) and drift tube temperature of 40 ℃. Result: Chemical compositions distribution of total flavonoids part focused on the fomer 25 min

but that of total saponins concentrated in after 25 min

purities of total flavonoids and total saponins were more than 50%

peak at 54.46 min was identified as betulinic acid. Conclusion: This preparation technology of total flavonoids and total saponins from Ziziphi Spinosae Semen is stable

which can be used as samples of pharmacodynamic experiment.