赤雹根中Dubioside F的分离纯化及含量测定

毛晓霞; 苗光新; 赵春颖; 佟继铭; 刘永平

doi:10.13422/j.cnki.syfjx.2014240043

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赤雹根中Dubioside F的分离纯化及含量测定

化学与分析 | 更新时间：2024-01-04

- 赤雹根中Dubioside F的分离纯化及含量测定
- Purification and Determination of Dubioside F in Tuber of
- 中国实验方剂学杂志 2014年20卷第24期页码：43-46
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省科技计划项目(14272502D);河北省高等学校自然科学研究青年基金项目(2010101);河北省重点学科建设项目
- DOI：10.13422/j.cnki.syfjx.2014240043
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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毛晓霞, 苗光新, 赵春颖, 等. 赤雹根中Dubioside F的分离纯化及含量测定[J]. 中国实验方剂学杂志, 2014,20(24):43-46.

MAO Xiao-xia, MIAO Guang-xin, ZHAO Chun-ying, et al. Purification and Determination of Dubioside F in Tuber of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 43-46.
毛晓霞, 苗光新, 赵春颖, 等. 赤雹根中Dubioside F的分离纯化及含量测定[J]. 中国实验方剂学杂志, 2014,20(24):43-46. DOI： 10.13422/j.cnki.syfjx.2014240043.

MAO Xiao-xia, MIAO Guang-xin, ZHAO Chun-ying, et al. Purification and Determination of Dubioside F in Tuber of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 43-46. DOI： 10.13422/j.cnki.syfjx.2014240043.

摘要

目的: 分离纯化赤雹根中的dubioside F

鉴定结构并测定不同产地赤雹根中dubioside F的含量. 方法: 通过硅胶柱色谱分离纯化

以CHCl3-MeOH-H2O (14:7:1)为洗脱剂

得到单体化合物

根据其理化性质和波谱数据

鉴定该单体化合物为dubioside F;再以分离得到的dubioside F为对照品

测定不同产地赤雹根药材中dubioside F的含量.采用Discovery-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水(24:76)

流速1.0 mL·min-1

检测波长200 nm. 结果: dubioside F在0.98~24.5 μg线性关系良好(r=1)

平均回收率96.96%

RSD 1.1%. 结论: 首次对赤雹根中单体皂苷类成分(dubioside F)进行含量测定

测定方法简便、快速、可靠.不同产地种植、野生的赤雹根药材dubioside F的含量

分别以吉林省和龙市、河北省宽城县出产的为最高.可为进一步的赤雹根药材质量控制的研究提供依据.

Abstract

Objective: The aim of this study was to purify and determine the content of dubioside F in the tuber of Thladiantha dubia from different habitats by HPLC Method: Dubioside F was purified by column chromatograph with silica gel(CHCl3-MeOH-H2O 14:7:1)and identified through physicochemical properties and spectral analysis. To determine the content of dubioside F in the tuber of T. dubia from different habitats by HPLC:A Discovery-C18 (4.6 mm×250 mm

5 μm) column was used with the mobile phase of acetonitrile-water (24:76). The DAD detector was set at 200 nm. Result: Dubioside F showed good linearity (r=1) within test ranges(0.98-24.5 μg). The average recovery was 96.96%

with RSD of 1.1%. Conclusion: This method was well validated in good precision

repeatability and sensitivity. The most abundant dubioside F in the tuber of T. dubia in different cultivated habitats was from Helong of Jilin rovince;while the most abundant dubioside F in the tuber of T. dubia in different natural habitats is from Kuancheng of Hebei province. This method was successfully applied to quantify dubioside F in the tuber of T. dubia.

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