Objective: The study aimed to establish the quality standards of Qiakekute granular. Method: Phellodendri Amurensis Cortex and Vaccariae Semen were identified by TLC. The contents of vaccarin

berberine hydro-chlorid

palmatine hydrochloride and ammonium glycyrrhizinate were determined by HPLC. Result: The TLC spots were clear and specific in good repeatability without inference from negative sample. The linear range of vaccarin was 7.204 0-108.059 3 mg·L-1(r=0.999 5) with an average recovery of 98.95%(RSD 2.2%). The linear range of berberine hydrochloride was 20.432 7-408.654 4 mg·L-1(r=0.999 6) with an average recovery of 101.83% (RSD 1.6%). The linear range of palmatine hydrochloride was 12.109 1-242.182 1 mg·L-1(r=0.999 5) with an average recovery of 98.24% (RSD 1.1%). The linear range of ammonium glycyrrhizinate was 0.061 08-0.407 2 g·L-1(r=0.999 5) with an average recovery of 100.10% (RSD 0.8%). The RSDs of precision

stability and reproducibility tests were all lower than 2%. Conclusion: The standard established in this study can be used for the quality control of Qiakekute granular.